[Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec085811 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec8517 [Full Text], Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec083378 Typically, protein samples are separated by SDS-PAGE and transferred to a nitrocellulose or PVDF membrane which is then probed with specific antibodies. [Full Text], Cold Spring Harb Protoc; 2011; doi:10.1101/pdb.rec12470 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec429 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec8458 [Full Text], Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.rec12188 18. [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10070 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11092 [Full Text], Transfer buffer for semi-dry electroblotting, Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10425 [Full Text], Cold Spring Harb Protoc; 2008; doi:10.1101/pdb.rec11517 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10018 [Full Text], Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.rec104901 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11067 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10369 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec10738 [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11761 Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 1986. [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec479 [Full Text], Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec085399 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec10711 [Full Text], Cold Spring Harb Protoc; 2009; doi:10.1101/pdb.rec11667 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec469 [Full Text], Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec082156 [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec083568 [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec10509 [Full Text], Cold Spring Harb Protoc; 2019; doi:10.1101/pdb.rec096701 After blocking with 5% bovine serum albumin in TBST buffer (137 mM NaCl, 20 mM Tris, and 0.5% Tween-20, pH 7.4) for 2 h at room temperature, the membrane was incubated with pan anti-lysine crotonylation antibody (PTM-502, 1:1000 dilution, PTM Biolabs) overnight at 4 °C. Adjust pH to 7.6 with 1 M HCl. [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec8645 The membrane was washed three times with TBST buffer for 5 min/wash. Tbst Recipe. [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec10725 [Full Text], Cold Spring Harb Protoc; 2014; doi:10.1101/pdb.rec085415 After blocking the membrane for 1 h at room temperature in 5% skim milk in TBST buffer (20 mM Tris–HCl [pH 7.5], 150 mM NaCl, and 0.1% Tween 20), the blot was probed with mouse monoclonal anti-HA primary antibody (Cell Signaling Technology) at a dilution of 1:1000 in the TBST buffer for 4 h at room temperature. [Full Text], Cold Spring Harb Protoc; 2006; doi:10.1101/pdb.rec8063 Acetate Buffer Particle Bombardment TBST Buffer ... E. F., and Shambrook, J. [Full Text], Cold Spring Harb Protoc; 2018; doi:10.1101/pdb.rec098780 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11062 © 2013 Cold Spring Harbor Laboratory Press, Alert me when Updates/Comments are published. 2. [Full Text], Cold Spring Harb Protoc; 2015; doi:10.1101/pdb.rec084509 [Full Text], Cold Spring Harb Protoc; 2010; doi:10.1101/pdb.rec12101 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec10971 [Full Text], Cold Spring Harb Protoc; 2007; doi:10.1101/pdb.rec11040 Autoclave and store at room temperature.